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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
13/04/2015 |
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Data da última atualização: |
19/06/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CERRO, P. del; ROLLA-SANTOS, A. A. P.; GOMES, D. F.; MARKS, B. B.; PÉREZ-MONTAÑO, F.; RODRÍGUEZ-CARVAJAL, M. A.; NAKATANI, A. S.; GIL-SERRANO, A.; MEGÍAS, M.; OLLERO, F. J.; HUNGRIA, M. |
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Afiliação: |
Pablo del Cerro; AMANDA ALVES PAIVA ROLLA SANTOS, CNPSo - Pós-graduanda; DOUGLAS FABIANO GOMES, CNPSo - Pós-graduando; BETTINA BERQUÓ MARKS, CNPSo - Pós-graduanda; FRANCISCO PÉREZ-MONTAÑO; MIGUEL ÁNGEL RODRÍGUEZ-CARVAJAL; ANDRÉ SHIGUEYOSHI NAKATANI; ANTONIO GIL-SERRANO; MANUEL MEGÍAS; FRANCISCO JAVIER OLLERO; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Regulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular signaling and host-legume nodulation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
BMC Genomics, London, [S. l.], v. 16, n. 251, Mar. 2015. 13 p. |
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ISSN: |
1471-2164 |
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DOI: |
10.1186/s12864-015-1458-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Nodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our proposed model considers that full nodulation of common bean by R. tropici requires both nodD1 and nodD2, whereas, in other legume species that might represent the original host, nodD1 plays the major role. In general, nodD2 is an activator of nod-gene transcription, but, in specific conditions, it can slightly repress nodD1. nodD1 and nodD2 play other roles beyond nodulation, such as swimming motility and IAA synthesis. MenosNodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our p... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogênio. |
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Thesaurus Nal: |
Nitrogen fixation. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122208/1/regulatory-nodD1.pdf
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Marc: |
LEADER 02861naa a2200289 a 4500
001 2013361
005 2017-06-19
008 2015 bl uuuu u00u1 u #d
022 $a1471-2164
024 7 $a10.1186/s12864-015-1458-8$2DOI
100 1 $aCERRO, P. del
245 $aRegulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular signaling and host-legume nodulation.$h[electronic resource]
260 $c2015
520 $aNodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our proposed model considers that full nodulation of common bean by R. tropici requires both nodD1 and nodD2, whereas, in other legume species that might represent the original host, nodD1 plays the major role. In general, nodD2 is an activator of nod-gene transcription, but, in specific conditions, it can slightly repress nodD1. nodD1 and nodD2 play other roles beyond nodulation, such as swimming motility and IAA synthesis.
650 $aNitrogen fixation
650 $aFixação de nitrogênio
700 1 $aROLLA-SANTOS, A. A. P.
700 1 $aGOMES, D. F.
700 1 $aMARKS, B. B.
700 1 $aPÉREZ-MONTAÑO, F.
700 1 $aRODRÍGUEZ-CARVAJAL, M. A.
700 1 $aNAKATANI, A. S.
700 1 $aGIL-SERRANO, A.
700 1 $aMEGÍAS, M.
700 1 $aOLLERO, F. J.
700 1 $aHUNGRIA, M.
773 $tBMC Genomics, London, [S. l.]$gv. 16, n. 251, Mar. 2015. 13 p.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
09/08/2019 |
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Data da última atualização: |
02/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
CAMARGO, A. P.; SOUZA, R. S. C. de; COSTA, P. de B.; GERHARDT, I. R.; DANTE, R. A.; TEODORO, G. S.; ABRAHÃO, A.; LAMBERS, H.; CARAZZOLLE, M. F.; HUNTEMANN, M.; CLUM, A.; FOSTER, B.; FOSTER, B.; ROUX, S.; PALANIAPPAN, K.; VARGHESE, N.; MUKHERJEE, S.; REDDY, T. B. K.; DAUM, C.; COPELAND, A.; CHENM U, M. A.; IVANOVA, N. N.; KYRPIDES, N. C.; PENNACCHIO, C.; ELOE-FADROSH, E. A.; ARRUDA, P.; OLIVEIRA, R. S. |
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Afiliação: |
ANTONIO PEDRO CAMARGO, Unicamp; RAFAEL SOARES CORREA DE SOUZA, Unicamp; PATRÍCIA DE BRITTO COSTA, Unicamp, University of Western Australia; ISABEL RODRIGUES GERHARDT, CNPTIA, Unicamp; RICARDO AUGUSTO DANTE, CNPTIA, Unicamp; GRAZIELLE SALES TEODORO, UFPA; ANNA ABRAHÃO, Unicamp, University of Western Australia; HANS LAMBERS, University of Western Australia; MARCELO FALSARELLA CARAZZOLLE, Unicamp; MARCEL HUNTEMANN, Department of Energy Joint Genome Institute; ALICIA CLUM, Department of Energy Joint Genome Institute; BRIAN FOSTER, Department of Energy Joint Genome Institute; BRYCE FOSTER, Department of Energy Joint Genome Institute; SIMON ROUX, Department of Energy Joint Genome Institute; KRISHNAVENI PALANIAPPAN, Department of Energy Joint Genome Institute; NEHA VARGHESE, Department of Energy Joint Genome Institute; SUPRATIM MUKHERJEE, Department of Energy Joint Genome Institute; T. B. K. REDDY, Department of Energy Joint Genome Institute; CHRIS DAUM, Department of Energy Joint Genome Institute; ALEX COPELAND, Department of Energy Joint Genome Institute; I.-MIN A. CHEN, Department of Energy Joint Genome Institute; NATALIA N. IVANOVA, Department of Energy Joint Genome Institute; NIKOS C. KYRPIDES, Department of Energy Joint Genome Institute; CHRISTA PENNACCHIO, Department of Energy Joint Genome Institute; EMILEY A. ELOE-FADROSH, Department of Energy Joint Genome Institute; PAULO ARRUDA, Unicamp; RAFAEL SILVA OLIVEIRA, Unicamp, University of Western Australia. |
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Título: |
Microbiomes of Velloziaceae from phosphorus-impoverished soils of the campos rupestres, a biodiversity hotspot. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Scientific Data, v. 6, p. 1-11, 2019. |
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DOI: |
https://doi.org/10.1038/s41597-019-0141-3 |
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Idioma: |
Inglês |
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Notas: |
Article number: 140. |
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Conteúdo: |
The rocky, seasonally-dry and nutrient-impoverished soils of the Brazilian campos rupestres impose severe growth-limiting conditions on plants. Species of a dominant plant family, Velloziaceae, are highly specialized to low-nutrient conditions and seasonal water availability of this environment, where phosphorus (P) is the key limiting nutrient. Despite plant-microbe associations playing critical roles in stressful ecosystems, the contribution of these interactions in the campos rupestres remains poorly studied. Here we present the first microbiome data of Velloziaceae spp. thriving in contrasting substrates of campos rupestres. We assessed the microbiomes of Vellozia epidendroides, which occupies shallow patches of soil, and Barbacenia macrantha, growing on exposed rocks. The prokaryotic and fungal profiles were assessed by rRNA barcode sequencing of epiphytic and endophytic compartments of roots, stems, leaves and surrounding soil/rocks. We also generated root and substrate (rock/soil)-associated metagenomes of each plant species. We foresee that these data will contribute to decipher how the microbiome contributes to plant functioning in the campos rupestres, and to unravel new strategies for improved crop productivity in stressful environments. |
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Palavras-Chave: |
Campos rupestres brasileiros; Condições extremas; DNA sequencing; Microbioma de plantas; Sequenciamento genético; Soils; Solos; Stressful environments. |
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Thesagro: |
Microrganismo. |
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Thesaurus NAL: |
Microbiome; Microorganisms; Velloziaceae. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/200538/1/AP-Microbiomes-Velloziaceae.pdf
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Marc: |
LEADER 02937naa a2200601 a 4500
001 2111238
005 2019-10-02
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41597-019-0141-3$2DOI
100 1 $aCAMARGO, A. P.
245 $aMicrobiomes of Velloziaceae from phosphorus-impoverished soils of the campos rupestres, a biodiversity hotspot.$h[electronic resource]
260 $c2019
500 $aArticle number: 140.
520 $aThe rocky, seasonally-dry and nutrient-impoverished soils of the Brazilian campos rupestres impose severe growth-limiting conditions on plants. Species of a dominant plant family, Velloziaceae, are highly specialized to low-nutrient conditions and seasonal water availability of this environment, where phosphorus (P) is the key limiting nutrient. Despite plant-microbe associations playing critical roles in stressful ecosystems, the contribution of these interactions in the campos rupestres remains poorly studied. Here we present the first microbiome data of Velloziaceae spp. thriving in contrasting substrates of campos rupestres. We assessed the microbiomes of Vellozia epidendroides, which occupies shallow patches of soil, and Barbacenia macrantha, growing on exposed rocks. The prokaryotic and fungal profiles were assessed by rRNA barcode sequencing of epiphytic and endophytic compartments of roots, stems, leaves and surrounding soil/rocks. We also generated root and substrate (rock/soil)-associated metagenomes of each plant species. We foresee that these data will contribute to decipher how the microbiome contributes to plant functioning in the campos rupestres, and to unravel new strategies for improved crop productivity in stressful environments.
650 $aMicrobiome
650 $aMicroorganisms
650 $aVelloziaceae
650 $aMicrorganismo
653 $aCampos rupestres brasileiros
653 $aCondições extremas
653 $aDNA sequencing
653 $aMicrobioma de plantas
653 $aSequenciamento genético
653 $aSoils
653 $aSolos
653 $aStressful environments
700 1 $aSOUZA, R. S. C. de
700 1 $aCOSTA, P. de B.
700 1 $aGERHARDT, I. R.
700 1 $aDANTE, R. A.
700 1 $aTEODORO, G. S.
700 1 $aABRAHÃO, A.
700 1 $aLAMBERS, H.
700 1 $aCARAZZOLLE, M. F.
700 1 $aHUNTEMANN, M.
700 1 $aCLUM, A.
700 1 $aFOSTER, B.
700 1 $aFOSTER, B.
700 1 $aROUX, S.
700 1 $aPALANIAPPAN, K.
700 1 $aVARGHESE, N.
700 1 $aMUKHERJEE, S.
700 1 $aREDDY, T. B. K.
700 1 $aDAUM, C.
700 1 $aCOPELAND, A.
700 1 $aCHENM U, M. A.
700 1 $aIVANOVA, N. N.
700 1 $aKYRPIDES, N. C.
700 1 $aPENNACCHIO, C.
700 1 $aELOE-FADROSH, E. A.
700 1 $aARRUDA, P.
700 1 $aOLIVEIRA, R. S.
773 $tScientific Data$gv. 6, p. 1-11, 2019.
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Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
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Registro completo
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Biblioteca(s): |
Catálogo Coletivo de Periódicos Embrapa; Embrapa Florestas; Embrapa Meio Ambiente; Embrapa Meio-Norte; Embrapa Trigo. |
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Identificador: |
69 |
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Data corrente: |
09/05/2002 |
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Data da última atualização: |
30/04/2015 |
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Código do título: |
0900016 |
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ISSN: |
0301-2123 |
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Código CCN: |
000690-4 |
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Título e Subtítulo: |
ACTA BIOLOGICA PARANAENSE |
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Entidade: |
Instituto de Biologia da Universidade Federal do Parana |
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Local de publicação: |
Curitiba, PR |
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Periodicidade: |
Irregural |
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Inicio de publicação: |
1972 |
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Coleções da unidade: |
Embrapa Florestas
1972 1(1/2,3/4); 1975 4(1/2,3/4); 1976 5(3/4); 1983 12(1/4); 1985 14(1/4); 1986 15(1/4); 1987 16(1/4); 1988 17(1/4); 1989 18(1/4); 1990 19(1/4); 1991 20(1/4); 1992 21(1/4); 1994 23(1/4); 1995 24(1/4); 1996 25(1/4); 1997 26(1/4); 1998 27(1/4); 1999 28(1/4); 2000 29(1/4); 2001 30(1/4); 2002 31(1/4); 2003 32(1/4); 2004 33(1/4); 2005 34(1/4); 2006 35(1/2,3/4); 2007 36(1/2,3/4); 2008 37(1/2,3/4); 2009 38(1/2,3/4); 2010 39(1/2,3/4); 2011 40(1/2,3/4); 2012 41(1/2,3/4)
Embrapa Meio Ambiente
1972/2012 1(1/2); 4 (1/2,3/4); 5(1/2,3/4); 6(1/4); 8; 7(1/4); 8(1/4); 9(1/4); 10(1/4); 11(1/4); 12(1/4); 13(1/4); 14(1/4); 15(1/4); 16(1/4); 17(1/4); 18(1/4); 19(1/4); 20(1/4); 21(1/4); 22(1/4); 23(1/4); 24(1/4); 25(1/4); 26(1/4); 27(1/4); 28(1/4); 29(1/4); 30(1/4); 31(1/4); 32(1/4); 33(1/4); 34(1/4); 35(1/2, 3/4); 36(1/4); 37(1/4); 38(1/4); 39(1/4); 40(1/4); 41(1/4)
Embrapa Meio-Norte
1972 1(1/2); 1985-2008 14-38; 2011 40(1-4); 2012 41(1/4)
Classificação: 574.05
Embrapa Trigo
1972/91 1 1972; 2 1973; 3 1974; 4 1975; 5 1976; 6 1977; 7 1978; 8 1979; 9 1980; 10 1981; 11 1982; 12 1983; 13 1984; 14 1985; 15 1986; 16 1987; 18 1989; 19 1990; 20 1991; 28(1-4) 1999.
Classificação: 574.05 |
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